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Imprint Immunofixation of IgG Subclasses, Using Monoclonal Subclass‐Specific Antibodies
Author(s) -
VARTDAL F.,
BIRD P.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb01809.x
Subject(s) - subclass , iif , immunofixation , monoclonal antibody , antibody , antiserum , isoelectric focusing , multiple myeloma , microbiology and biotechnology , immunology , monoclonal , biology , indirect immunofluorescence , biochemistry , enzyme
Monoclonal mouse hybridoma antibodies to human IgG subclasses 1–4 were used in an imprint immunofixation (IIF) technique to characterize the IgG subclass of a panel of IgG myeloma and normal sera separated by thin‐layer electrofocusing. The results show that the antisera were IgG subclass‐specific by this technique and that IIF can be a useful method in identifying IgG subclasses of IgG myeloma proteins in whole sera.