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T‐Cell Growth Factor Contains Activity that Supports Natural Killer Activity in Vitro
Author(s) -
GRÖNVIK K.O.,
ANDERSSON J.,
ALM G.,
STERN P.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb01802.x
Subject(s) - concanavalin a , cytotoxic t cell , lymphokine activated killer cell , in vitro , biology , natural killer cell , lysis , effector , cell , natural killer t cell , immunology , microbiology and biotechnology , interleukin 21 , biochemistry
Lymphocytes from athymic and normal mice were tested and compared for cytotoxic activity after in vitro cultures suppemented with concanavalin A or T‐cell growth factor (TCGF). Our results indicate that, in addition to cytoloxic T cells, natural killer (NK) activity can be recovered from lymphocytes cultured in the presence of TCGF and that this is not the result of contaminating interferon in the TCGF preparations. The NK nature of the effector cells was established by means of a panel of target cells, including a teratocarcinoma tumour cell, which enabled the distinction of T‐cell‐ and NK‐cell‐mediated lysis.