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Leu 7 + (HNK‐l + ) Cells
Author(s) -
PORWITKSIAZEK A.,
AMAN P.,
KSIAZEK T.,
BIBERFELD P.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00883.x
Subject(s) - percoll , cytotoxic t cell , monoclonal antibody , microbiology and biotechnology , biology , population , cell , cytotoxicity , immunofluorescence , lymphocyte , interleukin 21 , in vitro , immunology , antibody , biochemistry , medicine , environmental health
In the present study, combined methods (indirect immunofluorescence with monoclonal antibodies. Percoll density fractionation, FACS analysis, and the cytotoxicity test) were used for further characterization of peripheralblood Leu 7 + cells (human NK and K cells). The Leu 7 + cell content was found to be relatively higher in the low‐density cell fraction in which cells of large granular lymphocyte morphology predominated. However, Leu 7 + cells were also present in intermediate and high‐density fractions. Low‐density Leu 7 + cells were characterized by bothLeu 2 (T suppressor/cytotoxic) and OKM1 (myelomonocytic) markers, whereas among high‐density Leu 7 + cells the Leu 2 phenotype strictly predominated. Depletion of OKT 3 + cells from the non‐adherent cell population caused a decrease of cells with T helper and T suppressor phenotypes but did not have this effect on Leu 7 + and OKM1 + cells. After depletion of Leu 7 + cells from the OKT3 population the content of both T suppressor and OKM1 + cells decreased. Both the present results and previous reports enable us to conclude that two main Leu 7 + cell subpopulations are present in blood, namely Leu 7 + Leu 2 + /Leu 4 + and Leu 7 + /OKM 1 + cells. The presence of small and large Leu 7 + cells was also shown by FACSanalysis.