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Anti‐Oxazolone Hybridomas and Strain Distribution of the Oxazolone Idiotype
Author(s) -
McKIMMBRESCHKIN J. L.,
BRESCHKIN A. M.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00874.x
Subject(s) - idiotopes , idiotype , oxazolone , antiserum , antibody , hapten , radioimmunoassay , monoclonal antibody , microbiology and biotechnology , biology , balb/c , immunoglobulin idiotypes , titer , immunology , virology , immune system , biochemistry
Antibodies produced in the primary response to the hupten 2‐phenyl oxazolone (OX) express a eruss‐reactive idiotype in BALB/c and DBA/2 mice. We studied the response in hyperimmunized mice, using ascites produced after multiple immunizations with an OX conjugate and by generating monoclonal antibodies. A competitive radioimmunoassay was developed using a rabbit :tnti‐idiotype antiserum raised against purified hyperimmune anti‐OX antibodies, Mice from strains including CBA. C3H/He and B10.BR expressed all the deteminants found in BALB/c serum, although at a lower titre. C57BL/6 mice, however, only expressed some of the BALB/c determinants. We isolated a monoclonal antibody, 1F9, which expressed some of the determinants found in BALB'e serum. All the cross‐reactive idiotopes expressed on 1F9 were also expressed in the above strains including C57BL/6. Almost every BALB/c anti‐OX antibody also expressed the 1F9 determinants. There are thus determinants of the BALB/c OX idiotype which arc expressed in a number of different strains previously thought to be negative for the idiotype.