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Kinetic Analysis of Subset Growth and B‐Cell Activation in Pokeweed Mitogen‐Stimulated Cultures of Peripheral Blood Mononuclear Cells Depleted of or Enriched for OKT8 + Cells
Author(s) -
JONES B. M.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00871.x
Subject(s) - pokeweed mitogen , peripheral blood mononuclear cell , microbiology and biotechnology , biology , stimulation , cell culture , b cell , peripheral blood , immunology , antibody , in vitro , endocrinology , biochemistry , genetics
Peripheral blood mononuclcar cells (PBMN) that were depleted of OKT8 + cells and stimulated with pokeweed mitogen (PWM) produced higher cell yields and higher numbers of plaque‐forming cells than unfractionated PBMN. Conversely, OKT8‐enriched PBMN, prepared by mixing unfractionated and OKT8 + cells in a ratio of 3:1, gave reduced cell growth and B‐cell activation. In OKT8‐depleted cultures, B cells, OKT4 + cells, OKT8 + cells, and OKM1 + cells increased in number between days 4 and 7 of culture by factors of 9.8, 5.9, 20.1, and 5.6 respectively, whereas growth rates for these subsets were 2.4, 1.0, 2.0, and 1.3 in unfractionated cultures and 0.9, 1.0, 1.2, and 0.6 in cultures enriched for OKT8 + cells. On day 7 of culture, 73±10% of B cells secreted immunoglobulin in unfractionated cultures, whereas only 21±10% of B cells were activated in OKT8‐enriched cultures. Surprisingly, PWM stimulation of OKT8‐depleted PBMN produced only 40±12% activated B cells.

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