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Agar Accumulates in Rat Peritoneal Macrophages Elicited with Thioglycollate Broth
Author(s) -
EICHNER R. D.,
SMEATON T. C.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00866.x
Subject(s) - agar , microbiology and biotechnology , polysaccharide , ingestion , glycogen , biology , anthrone , agar plate , bacteria , chemistry , biochemistry , genetics
Thioglycollate broth‐ and agar‐elicited rat peritoneal cxudate cells contain copious amounts of agar, a sulphated polysaccharide contained in the eliciting agent; the agar content has been measured chemically by the anthrone method for neutral polysaccharides. Adherence experiments confirm that day‐3 thioglycollate‐elicited macrophages coniain 95% of the measured agar; on day 1, 72% is associated with polymorphonuclear cells. Agar ingestion by macrophages coincides with increases in glycogen synthase activity. We suggest that ingestion of agar may be related to the reported deficiencies in function of thioglycollate‐elicited or treated macrophages.