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Functional Relationship of Human T Lymphocytes, Monocytes and Interleukins
Author(s) -
ULMER A.J.,
FLAD H.D.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00850.x
Subject(s) - phytohaemagglutinin , biology , differential centrifugation , monocyte , stimulation , immunology , interleukin 3 , t cell , lymphokine , interleukin 2 , microbiology and biotechnology , centrifugation , interleukin , cytokine , interleukin 21 , immune system , endocrinology , biochemistry
The different requirements of human T lymphocytes of different densities for accessory cells and helper factors (Interleukin 1 (Il‐1) and Interleukin 2 (Il‐2)) in the response to phytohacmagglutinin (PHA) were investigated. Humun T lymphocytes were subfractionated by discontinuous density gradient centrifugation. The various T‐cell subsets were stimulated by PHA to form colonies in an agar micro‐culture in the presence or absence of additional adherent cells or crude preparations of Il‐1 or Il‐2. The results show that the higher the density of the fractionated T lymphocytes and the lower the number of cells cultured, the greater is the number of adherent cells or the amount of helper factors required for the stimulation of colony‐forming T lymphocytes. The results are consistent with the assumption that monocytes provide positive modulating activity during mitogenic stimulation of colony‐forming T lymphocytes. The number of monocytes necessary for exerting an optimal modulating activity depends on the number of T cells cultured and the density of the T‐cell fraction. This may reflect a distinct susceptibility of T cells of different densities to monocyte‐mediated helper effects.