Premium
Analysis of Leptospira Antigens by Crossed Immunoelectrophoresis
Author(s) -
TERPSTRA W. J.,
SCHOONE G. J.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00847.x
Subject(s) - serotype , antigen , leptospira , immunoelectrophoresis , leptospira interrogans , leptospirosis , biology , virology , microbiology and biotechnology , immunology
Serovars icterohaemorrhagiae, canicola , and arboreae of pathogenic Leptospira interrogans and serovar patoc of saprophytic L. biflexa were examined by crossed immunoelectrophoresis. A close antigenic relationship was found between the interrogans serovars. particularly between icterohaemorrhagiae and canicola . To a much lesser extent cross‐reactions were found between interrogans serovars and patoc . Comparison of three different antigenic preparations of icterohaemorrhagiae by tandem crossed immunoelectrophoresis and by absorption experi ments with the patoc reference system showed the presence of several common broadly reactive antigens. Antigen I of the reference system, a genus‐specific heat‐stable protein antigen, was found in all antigenic preparations. It is a diagnostically important antigen.