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Simultaneous Determination of the Concentration and Lytic Activity of Effector Cells that Mediate Natural and Antibody‐Dependent Cytotoxicity
Author(s) -
CALLEWAERT D.M.,
GENYEA J.,
MAHLE N. H.,
DAYNER S.,
KORZENIEWSKI C.,
SCHULT S.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00815.x
Subject(s) - cytotoxicity , lytic cycle , effector , lysis , lymphocyte , chemistry , natural killer cell , enzyme , biophysics , microbiology and biotechnology , biology , biochemistry , in vitro , immunology , virus
Cellular cytotoxicity reactions can be studied in a manner analogous to that used to measure enzyme activity. This approach yields two parameters; V max the maximal rate of target cell lysis that can be achieved by the lymphocyte preparation tested, and K M app , the apparent Michaelis constant. By analogy to many enzyme‐catalysed reactions, K M app values for cytotoxicity reactions have generally been interpreted in terms of dissociation constants for the interaction of receptor sites on effector cells with antigens on the target cells, In this paper we demonstrate that experimentally determined K M app values for natural or antibody‐dependent cytotoxicity reactions are approximately equal to the concentration of NK or K. effector cells in the lymphocyte preparation tested. This result makes possible the simultaneous determination of both effector cell frequency and lytic activity in a given lymphocyte preparation.