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Tumour‐Host Interactions in Vivo
Author(s) -
GAUDERNACK G.,
OLSTAD R.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00790.x
Subject(s) - ascites , spleen , in vivo , in vitro , macrophage , population , cell , intraperitoneal injection , peritoneal fluid , biology , pathology , chemistry , immunology , endocrinology , medicine , biochemistry , microbiology and biotechnology , environmental health
We have earlier shown that coculture of macrophages and cells from a methylcholanthiene‐induced sarcoma (MCI M‐AA) in vitro resulted in macrophage activation and production of type II (gamma) interferon. When ascites fluid from the MCIM‐AA sarcoma (shown previously to activate macrophages in vitro) was added to spleen cell populations from semisyngeneic C 3 D 2 mice in vitro. NK activity was markedly enhanced. After intraperitoneal injection of MCI M‐AA cells or ascites fluid, 4‐ to 12‐fold increased NK cell activity with a peak at 3–5 days could be measured in the spleen cell population and in the non‐adherent peritoneal exudate cell population. The mice injected with tumour cells or ascites fluid developed a pronounced splenomegaly, and maximum spleen size coincided with peak NK activity. Injection of tumour cells or tumour ascites fluid resulted in marked changes in the T‐cell, B‐cell, macrophage, and ‘null’ cell content of ihe peritoneal exudate.