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Ox Erythrocyte Cytotoxicity by Phorbol Myristate Acetate‐Activated Human Neutrophils
Author(s) -
DALLEGRI F.,
PATRONE F.,
BONVINI E.,
GAHRTON G.,
HOLM G.,
SACCHETTI C.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00772.x
Subject(s) - phorbol , cytolysis , superoxide dismutase , catalase , myeloperoxidase , respiratory burst , lysis , hemolysis , cytotoxicity , chemistry , chronic granulomatous disease , biochemistry , hydrogen peroxide , microbiology and biotechnology , neutrophile , cytotoxic t cell , reactive oxygen species , superoxide , granulocyte , biology , immunology , oxidative stress , in vitro , protein kinase c , enzyme , inflammation
Human neutrophils activated by phorbol myristatc acelatc were cytoioxic lo ox erythrocyles. as determined by the 51 Cr release method. Maximal cytolysis was obtained with a phorbol myristute acetate concentration of 5 ng/ml and with an etTcctor to target cell ratio of 1:4. An inlact neutrophil metabolic burst and production of oxygen‐dcrived‐frec radicals were essential lor the cytotnxic event, since neulrophils from patients with chronic granulo‐malous disease failed to exhibit any ox erythrocyte lysis. The target cell destruction was completely prevented by catalase, was unaffected by supcroxidc dismutase. and was reduced approximately to one‐third by azide and cyanide. These data suggest that, under our experimental conditions, the ox erythrocyte killing by phorbol myristatc acetate‐activated neutrophils mainly depends on myelopcroxidasc and hydrogen peroxide.