Premium
Two Distinct Azobenzenearsonate‐Specific Helper T‐Cell Subpopulations Mediate Different Forms of T‐B Cooperation
Author(s) -
ADORIMI L.,
AGAROSSI G.,
FIORAVANTI D.,
DORIA G.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00771.x
Subject(s) - priming (agriculture) , biology , t cell , cytotoxic t cell , microbiology and biotechnology , interleukin 21 , in vitro , antigen presenting cell , cd40 , keyhole limpet hemocyanin , immunology , immune system , biochemistry , botany , germination
Popliteal lymph node T cells from mice footpad‐primed with azobenzenearsonate (ABA)‐protein conjugates were able to help the anti‐trinitrophenyl (TNP) and anti‐ABA plaque‐forming cell (PFC) responses of normal syngeneic spleen cells cultured in vitro with TNP‐ABA‐keyhole limpet haemocyanin. Enrichment in ABA‐specific helper cells was obtained by positive selection of ABA‐primed T cells on ABA‐pulsed syngeneic macrophages. The ABA‐specific T cells induced by ABA‐protein priming are able to help the anti‐TNP PFC response of normal B cells through recognition of the ABA determinant either unlinked to TNP (ABA and TNP separately presented to T and B cells) or linked to TNP (ABA and TNP presented as moieties of the same molecule). These two mechanisms of T‐B cooperation are mediated by two different ABA‐specific helper T‐cell subpopulations, which can be distinguished by their different radiosensitivities: the former mechanism is mediated by radioresistant T cells, whereas the latter is mediated by radiosensitive T cells. Both helper T‐cell subpopulations bind the ABA‐pulsed syngeneic macrophages, demonstrating the presence of ABA‐specific receptors on both cell types.