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Cytochemical Analysis of Human Peripheral Blood Lymphocyte Subsets Defined by Monoclonal Antibodies
Author(s) -
BERNARD J.,
DUFER J.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00769.x
Subject(s) - monoclonal antibody , cytotoxic t cell , microbiology and biotechnology , antibody , esterase , lymphocyte , inducer , t lymphocyte , enzyme , chemistry , monoclonal , peripheral blood , biology , immunology , biochemistry , antigen , in vitro , gene
Human blood lymphocyte subpopulaiions, revealed by a panel of commercially available monoclonal antibodies by means of a resetting technique, were submitted to direct cyto‐chemicat analysis and were shown to have distinguishing characteristics. T cells reactive with OKT3 antibody (T3 + ) displayed higher beta‐glucuronidase and dot‐like alpha‐napnthyl acetate acid esterase (ANAE) activity than T3 − cells. Helper/inducer cells (T4 + ) were characterized by a high level of dot‐like ANAE activity, whereas cytotoxic/suppressor cells (T8 + ) displayed selective naphthol‐ASD‐chloroacetate esterase activity. These results provide evidence for association of some cytoplasmic enzyme activities with the expression of membrane differentiation markers defined by monoclonal antibodies.