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Characterization of Effector Cells Mediating IgG and IgM Antibody‐Dependent Cellular Cytotoxicity
Author(s) -
ZÖLLER M.,
ANDRIGHETTO G. C.,
HEYMAN B.,
LAMON E. W.,
WIGZELL H.
Publication year - 1983
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1983.tb00761.x
Subject(s) - cytolysis , antibody dependent cell mediated cytotoxicity , antibody , microbiology and biotechnology , immunoglobulin m , cytotoxicity , immunoglobulin g , biology , effector , immunology , monoclonal antibody , chemistry , in vitro , biochemistry
Spleen effector cells for IgG‐ and IgM‐induced antibody‐dependent cellular cytotoxicity (ADCC) were characterized with respect to density and cell surface markers by using sheep erythrocytes (SRBC) coated with hybridoma‐derived monoclonal anti‐SRBC IgG or anti‐SRBC IgM antibodies as targets. While basically the same effector celts are cytolytic for IgG and IgM antibody‐coated SRBC, they differ with respect to their relative killing capacity for IgG‐ versus IgM‐coated target cells. On the basis of physical and biochemical properties three populations with cytolytic capacity could be separated: (I) A light fraction of large cells had high cytolytic potential for both IgG‐ and IgM‐coated SRBC. The cells were negative for the Fc receptor for IgG (Fcy‐R − ) and the C3‐receptor (C3‐R − ), they carried the receptor for Helix pomatia A agglutinin (HP‐A + ), and reactivity was strongly reduced after treatment with anti‐Thy‐1 and complement (C). (II) High activity was also observed with a medium‐dense fraction, preferably lysing IgG antibody‐coated cells. The cells were Fcy‐R + , partly C3‐R + , mostly HP‐A − , and only a minor portion of the cells were Thy‐1 + . (Ill) A dense fraction, displaying on a percell basis low cytolytic potential, was more active in IgM than IgG ADCC. The cells were Fcy‐R + , HP‐A + and Thy‐1 + . All three effector cell populations were non‐adherent, non‐phagocytic, and surface immunoglobulin‐negative (s‐Ig − ).