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Ribonucleic Acid Antigen from the Reiter Treponeme Used in an ELISA for Antibodies in Syphilis
Author(s) -
PEDERSEN N. STRANDBERG,
PETERSEN C. SAND,
AXELSEN N. H.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00743.x
Subject(s) - syphilis , treponema , antigen , antibody , virology , chemistry , flagellum , rna , microbiology and biotechnology , primary syphilis , medicine , biology , immunology , biochemistry , gene , human immunodeficiency virus (hiv)
Purified RNA from Treponema phagedenis biotype Reiter was used as antigen in an enzyme‐linked immunosorbent assay (ELISA) for IgG antibodies in syphilis. The RNA ELISA was compared with the fluorescent treponemal antibody absorption test (FTA‐ABS), the Treponema pallidum immobilization test (TPI), and with two ELlSAs using purified flagellum from the Reiter treponeme as antigen (flagellum ELISA), and sonified Reiter treponeme culture as antigen (sonicate ELISA). A total of 729 sera from patients with and without syphilis were studied. The RNA ELISA had a lower sensitivity ( P < 0.01) in primary syphilis than the ftagellum ELISA, the sonicate ELISA, and the FTA‐ABS. In treated syphilis the RNA ELISA was also less sensitive than the TPI ( P < 0.01).