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Quantitation of Cells Secreting Immunoglobulins after Elution from Rheumatoid Synovial Tissue
Author(s) -
EGELAND T.,
LEA T.,
MELLBYE O. J.,
PAHLE J. A.,
OTTESEN T.,
NATVIG J. B.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00741.x
Subject(s) - rheumatoid arthritis , incubation , peripheral blood mononuclear cell , antibody , in vivo , incubation period , synovial membrane , synovial fluid , pathology , chemistry , immunology , medicine , biology , in vitro , biochemistry , alternative medicine , microbiology and biotechnology , osteoarthritis
Mononuclear cells (MNC) eluted from rheumatoid synovial tissue of 16 patients with rheumatoid arthritis were examined for immunoglolulin‐secreting cells (ISC). Immediately after elution and separation synovial tissue MNC contained considerable numbers of ISC. IgG and IgA ISC were more abundant than IgM ISC. At the same time there were low numbers of ISC in blood. Synovial tissue ISC were lost during incubation with pokeweek mitogen (PWM), possibly because tissue MNC were already maximally triggered in vivo. This was in contrast to blood MNC, in which the number of ISC increased significantly during incubation with PWM.