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The Localization of Glomerular C3b Receptor by Immunoelectron Microscopy
Author(s) -
MATRE R.,
NILSEN R.,
THUNOLD S.,
TØNDER O.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00734.x
Subject(s) - immunoelectron microscopy , immunoperoxidase , basement membrane , antiserum , cytoplasm , staining , stain , glomerular basement membrane , pathology , electron microscope , biology , chemistry , cytochemistry , microbiology and biotechnology , kidney , ultrastructure , antigen , anatomy , immunohistochemistry , antibody , glomerulonephritis , immunology , monoclonal antibody , medicine , endocrinology , physics , optics
By an indirect immunopcroxidase technique, rabbit antiserum to solubilizcd C3b receptor stained the glomeruli in cryostat sections of human kidney. The staining was localized to the glomerular basement membrane zone. Glomcruli in sections of aldehyde‐fixed paraffin‐embedded tissue did not stain. However, if the sections were pretreatcd with proteolytic enzymes, the glomeruli were stained by the antiserum as in the cryostat sections. Electron microscopy revealed that the reaction product was located in the cell membrane of the epithelial cells. In some cells the reaction product was also found in vacuoles in the cytoplasm. The basement membrane and endothelial and mesangial cells did not stain.