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Migration of Prebursal Stem Cells from the Early Chicken Embryo to the Yolk Sac
Author(s) -
LASSILA O.,
MARTIN C.,
DIETERLENLIÈVRE F.,
GILMOUR D. G.,
ESKOLA J.,
TOIVANEN P.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00722.x
Subject(s) - yolk sac , embryo , incubation , biology , andrology , chimera (genetics) , stem cell , yolk , anatomy , microbiology and biotechnology , genetics , medicine , gene , biochemistry , ecology
Allogeneic yolk sac—embryo chimaeras were constructed by association of S 15 B 15 yolk sac and B 2 B 2 embryo on day 2 of incubation. Five days later yolk sac cells from the chimaeras were injected intravenously into 14‐day‐old irradiated embryos, using recipients of B 2 B 2 and B 15 B 15 genotypes. One week after hatching, cells in the bursa of Fabricius and peripheral blood erythrocytes were studied for la‐like antigens and B alloantigens, respectively, to determine whether they were derived from the embryo or yolk sac part of the chimaera. The results obtained demonstrate that prebursal and erythropoietic stem cells migrate from the early embryo to the yolk sac during the 2nd to the 7th day of incubation. They also exclude the de novo generation of prebursal stem cells in the yolk sac.