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In Vivo Collaboration between Precursor T Cells and Helper T Cells in the Development of Delayed‐Type Hypersensitivity Reaction to Influenza Virus in Mice
Author(s) -
LEUNG K. N.,
SCHILTKNECHT E.,
ADA G. L.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00721.x
Subject(s) - spleen , virus , immune system , in vivo , immunology , hypersensitivity reaction , biology , antigen , t cell , concanavalin a , virology , in vitro , microbiology and biotechnology , biochemistry
Nude, athymic mice do not mount a delayed‐type hypersensitivity (DTH) response to influenza A virus. A single injection of T helper cells (γ‐irradiated, 2‐day immune spleen cells) or three injections over 3 days of a concanavalin‐A‐activated spleen cell supernatant to virus‐sensitized nude mide resulted in a ‘normal’ DTH response when the mice were challenged with the virus. It was previously shown that the cells responsible for the reaction were T cells and required I‐region compatibility. Injection of T helper cells into normal mice did not affect the level of the subsequent DTH response. However, injection of such cells into mice pretreated with anti‐thymocyte serum (ATS) restored the ability of the mice to mount a DTH response. The results show that (1) nude mice contain precursor T cells for influenza virus antigen; (2) an I region‐restricted response can be generated in the absence of a thymus; and (3) in vivo collaboration between DTH T‐cell precursors and helper T cells can be shown to occur in congenitally nude mice and ATS‐treated mice.