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Density Distribution Profiles of T Cells: T M , T G and T A Cells and Response Patterns in Autologous Versus Allogeneic MLRs
Author(s) -
CAICA G. W.,
KABELITZ D.,
SJÖBERG O.,
AIGRAIN Y.,
WIGZELL H.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00719.x
Subject(s) - percoll , peripheral blood , immune system , population , microbiology and biotechnology , peripheral blood mononuclear cell , immunology , fractionation , chemistry , biology , medicine , biochemistry , centrifugation , in vitro , chromatography , environmental health
Discontinuous Percoll gradients have been used to obtain selected humanperipheral blood T lymphocytes without having to resort to interactions with immune complexes in the fractionation of T M , T G and T A cells. Here, we could show that T A cells represent a heterogeneous population with no distinct density profile, in contrast to light(T G ) and heavy (T M ) cells. Enriched, heavy T M cells could be shown to be excellent responders in allogeneic MLR while failing to react in autologous MLR In contrast, T cells of light density preferentially respond in autologous compared with allogeneic MLR.