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Affinity and Inhibitory Capacity of T‐Cell Proliferation of Monoclonal Anti‐Ia Antibodies
Author(s) -
LEMONNIER F. A.,
BIRNBAUM D. Z.,
DUBREUIL P. C.,
CAILLOL D. H.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00718.x
Subject(s) - monoclonal antibody , antigen , antibody , spleen , microbiology and biotechnology , monoclonal , inhibitory postsynaptic potential , epitope , mixed lymphocyte reaction , biology , population , t lymphocyte , immunology , chemistry , t cell , immune system , medicine , endocrinology , environmental health
The rates of dissociation from the surface of la‐positive spleen cells of three different anti‐Ia monoclonal antibodies were evaluated and compared with their inhibitory effects on T‐ lymphocyte proliferate responses involving the same spleen cell population, either as a source of accessory cells (responses to soluble antigens) or as allogeneic stimulating cells. In general, a direct relationship was observed between the affinity with which these monoclonal antibodies interact with Ia‐positive cells and the magnitude of their inhibitory effects, whether soluble antigen‐ or alloantigen‐induced responses were tested. These results indicate that the affinity of monoclonal antibodies is a factor that has to be considered to the same extent as their fine specificity when interpreting the results of inhibitory studies of T‐cell responses by anti‐Ia monoclonal antibodies.