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Lymphoid Cell Lines as Indicators of Lymphokine Generation
Author(s) -
CULBERT E. J.,
COCHRAN A. J.,
CLEMENTS G. B.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00709.x
Subject(s) - phytohaemagglutinin , lymphokine , cell culture , splenocyte , microbiology and biotechnology , population , immunology , biology , peripheral blood , medicine , antibody , antigen , genetics , environmental health
We investigated the migration characteristics of the cells of four human lymphoid lines, normal peripheral blood leucocytes (PBL), and normal mouse splenocytes (MSC). Two lines (QIMR‐WIL and Namalwa) were actively migratory, as were the PBL and MSC. Migration was inhibited at low temperatures and reduced by inhibitors of glycolysis, oxidative phosphorylation and RNA synthesis. The migration of QIMR‐WIL cells, PBL and MSC but not Namalwa was inhibited by lymphokine‐containing supernatants from phytohaemagglutinin‐pulsed MSC or MSC stimulated by allogeneic cells. The inhibitor of migration in the supernatants had properties similar to those of human leucocyte inhibition factor but distinct from those of macrophage inhibition factor. QIMR‐WIL and normal human PBL were compared as indicators in an indirect leucocyte migration assay. QIMR‐ WIL cells were the more sensitive responder cells and are an abundant, stable, and uniform cell population for lymphokine detection.