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Autologous Mixed Lymphocyte Reaction in Man
Author(s) -
DAMLE N. K.,
GUPTA S.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00699.x
Subject(s) - monoclonal antibody , mixed lymphocyte reaction , interleukin 21 , cytotoxic t cell , t lymphocyte , t cell , antigen presenting cell , microbiology and biotechnology , immunology , biology , antibody , immune system , in vitro , biochemistry
Human peripheral T cells and T‐cell Subpopulations defined with monoclonal antibodies of the OKT series were studied for the proliferative response on stimulation with autologous non‐T cells or mitogen‐activated T cells as stimulators in autologous mixed lymphocyte reaction (AMLR). T cells exhibited a vigorous proliferative response when stimulated with autologous non‐T cells or activated T cells but not with unactivated T cells. The stimulatory capacity of non‐T cells or activated T cells in AMLR was inhibited by prior treatment of stimulator cells with 7, 2 anti‐human Ia framework‐specific monoclonal antibody in the absence of complement. A BUdR and light technique was used to ablate proliferating T cells in response to either non‐T cells or activated T cells in AMLR. The removal of T cells responding to autologous non‐T cells left the responsiveness to autologous activated T cells relatively intact. Conversely, the removal of T cells responding to autologous activated T cells left the responsiveness 10 autologous non‐T cells relatively intact. Thus T cells responding to autologous non‐T cells appear to be distinct from those responding to autologous activated T cells in AMLR. Further analysis with OKT4 and OKT8 monoclonal antibodies showed that the major population responding to autologous non‐T cells was contained in OKT4 + T cells and that responding to autologous activated‐T cells was contained in OKT8 + T‐cell subset. However, both OKT4 + and OKT8 + T‐cell subsets responded by proliferation to non‐T cells and activated T cells in allogeneic MLR. T cells selected after AMLR between T and non‐T cells or T and activated T cells were treated with mitomycin and examined for their regulatory influence on the proliferative response of fresh autologous responder T cells. T cells selected from T and non‐T AMLR augmented and those obtained from T and activated T AMLR suppressed the proliferative responses of fresh autologous T cells to phytohaemagglutinin and to autologous or allogeneic non‐T cells in AMLR or allogeneic MLR. These findings indicate that in AMLR between T and non‐T cells or T and activated T cells phenotypically distinct Subpopulations of T lymphocytes respond by proliferation and express distinct immunoregulatory functions.