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Role of the E k Molecule in the Generation of Suppressor T Cells in Response to LDH B
Author(s) -
BAXEVANIS C. N.,
ISHII N.,
NAGY Z. A.,
KLEIN J.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00695.x
Subject(s) - priming (agriculture) , microbiology and biotechnology , t cell , chemistry , cd40 , lymph node , antigen presenting cell , antigen , monoclonal antibody , in vitro , biology , antibody , immunology , cytotoxic t cell , immune system , biochemistry , botany , germination
The role of the E k (E α E β k ) molecule in the generation of suppressor T (T s ) cells specific for lactate dehydrogenase B (LDH B ) was studied using different approaches. First, lymph node cells from LDH B ‐primed B1O.A(2R) (A k E k ) nonresponder mice were shown to suppress the LDH B ‐specific and A k ‐restricted proliferative response of T cells from the congenic responder strain B10.A(4R), which does not express E molecules (A k E o ). Similarly, lymph node cells from primed CBA (A k E k ) mice suppressed the anti‐LDH B response of Lyt‐1 + Lyt‐2 ‐ T celfs (depleted of Lyt‐2‐bearing T S cells) from the same mice. Second, in vitro priming of 2R (A k E k ) T cells with LDH B ‐pulsed 4R (A k E o ) antigen‐presenting cells (APC) generated T‐cell proliferation but not suppression. Third, nonresponder 2R mice were turned into responders by injecting them with LDH B ‐pulsed 4R APC or monoclonal Ia.m7 antibody that blocks the E k molecule. The data demonstrate that expression of E k molecules by the APC is necessary to generate LDH B ‐specific T s cells, which in turn prevent the proliferation of Lyt‐l + Lyt‐2 ‐ (probably helper) cells recognizing the same antigen in the context of the A k molecule.