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Studies on Mouse Auto‐reactive Cells
Author(s) -
CHARREIRE J.,
BACH J. F.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00692.x
Subject(s) - cytotoxicity , spleen , cytotoxic t cell , population , lymphatic system , biology , immunology , lymph , microbiology and biotechnology , in vitro , ficoll , pathology , chemistry , medicine , biochemistry , peripheral blood mononuclear cell , environmental health
In mice, an in vitro cell‐mediated cytotoxicity is directed towards syngeneic erythrocytes used as targets. This phenomenon occurs in lymphoid organs of normal, non‐immunized mice. Its intensity is greater in the thymus than in spleen or lymph nodes. The study of the nature of the cell responsible for this spontaneous syngeneic cytotoxicity ruled out the role of macrophages. The involvement of T cells in this phenomenon is assessed by its disappearance after the lymphoid celts had been treated with anti‐theta serum plus complement, by its disappearance from lymphoid organs of mice previously treated by hydrocortisone, and by its decrease in the presence of synthetic thymic factor. Moreover, spontaneous syngeneic cytotoxicily is lost when lymphoid cells are depleted of autologous rosettes by centrifugation on a Ficoll‐Hypaque gradient after rosette formation. Cytotoxic lymphocytes might belong to the population of auto‐rosettes previously characterized as a population of immature T cells.