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Release of Mediators from Purified Rat Mast Cells during Phagocytosis
Author(s) -
CZARNETZKI B. M.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00687.x
Subject(s) - phagocytosis , mast (botany) , immunology , chemistry , mast cell , microbiology and biotechnology , biology
Purified mature rat peritoneal mast cells, on exposure to zymosan or latex beads, phagocytize these particles, although less efficiently than macrophages. During phagocytosis, histamine, β‐glucuronidase, and eosinophil chemotactic factor are released from mast cells in a time‐, temperature‐ and dose‐dependent fashion. Complement components, cytochalasin B (5 μg/m1, and indomethacin (10 ‐6 m). enhanced mediator release, whereas compound BW 755C (20 μg/ml), a cyclooxygenasc and lipoxygenase inhibitor of arachidonate metabolism, totally abolished this process. Phagocytosis of mast cells thus activates imracellular mechanisms that closely resemble those observed with other phagocytic cells. These observations add a new perspective to the role of mast cells in inflammatory events.