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A Synergistic Polyclonal Response to Dextran Sulphate and Lipopolysaccharide
Author(s) -
BERGSTEDTLINDQVIST S.,
FERNANDEZ C.,
SEVERINSON E.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00669.x
Subject(s) - lipopolysaccharide , polyclonal antibodies , secretion , dextran , cell culture , cell growth , microbiology and biotechnology , antibody , biology , chemistry , immunology , biochemistry , genetics
The effects of the simultaneous addition of lipopolysaccharide (LPS) and dextran sulphate (DxS) were studied in a low‐cell density culture system, which enables exlensivc cell proliferation and high immuoglobulin secretion. Using these two milogens, a synergistic‐response was observed, with regard to both cell division und IgM secretion. However, only a very low IgG production could be detected. This was caused by the extensive cell proliferation. leading to suboptimal culture conditions. Thus, when the blasts, were recultured at a lower cell density or when a lower initial cell concentration was used, a high IgG response was obtained. The synergistic response induced by LPS plus DxS was independent of T cells, Furthermore, no apparent need for phagocytic cells was found. Both the LPS‐ and the LPS plus DxS‐induced activation led to a switch, preferentially to IgG2b and IgG3 secretion. This subclass pattern was not changed when the cultures were kicking functional T cells.