Premium
The Cytotoxic Effect of Mouse Macrophages Stimulated in Vitro by a β‐1,3‐ d ‐Glucan from Yeast Cell Walls
Author(s) -
BÖGWALD J.,
JOHNSON E.,
SELJELID R.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00652.x
Subject(s) - mastocytoma , cytolysis , cytotoxic t cell , cytotoxicity , cell culture , in vitro , macrophage , glucan , cell , stimulation , chemistry , cell division , microbiology and biotechnology , lysis , biology , biochemistry , genetics , neuroscience
Macrophages stimulated by an insoluble β‐1,3‐ d ‐glucan from yeast cell walls were able to destroy tumour cells as measured hy the release of radioactive label from prelabelled 14 C‐thymidine cells. Target cells were B‐16 melanoma. P‐815 rrmstocytoma, and the L‐929 cell line, A significant target cell killing by macrophages stimulated by glucan was observed after 72–96 h. The cytolysis of L‐929 cells was investigated in some detail. No stable soluble cytolytic factor appeared to be released into the medium during the stimulation of macrophages by glucan. since cell‐free spent medium had no cytotoxic effect on L‐929 cells. The densities of the macrophage monolayers were critical for an effective target cell killing; dense cultures showed more cyioioxicity than less dense cultures. The kinetics of the development of macrophagc‐mediated cytotoicit suggests a minimum stimulation period of 4 days for maximal cylolysis.