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Interleukin‐2 and Serum Thymic Factor Enable Autologous Rosette‐forming T Lymphocytes to Generate Helper and Cytotoxic Functions
Author(s) -
SUGAWARA F.,
PALACIOS R.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00644.x
Subject(s) - cytotoxic t cell , monoclonal antibody , cytotoxicity , microbiology and biotechnology , biology , immunology , interleukin , interleukin 3 , interleukin 2 , chemistry , antibody , interleukin 21 , t cell , immune system , in vitro , cytokine , biochemistry
The autologous rosette‐forming T cells (Tar cells) isolated by means of their ability to form rosettes with autologous erythrocytes were characterized by the use of OKT monoclonal anti‐human T‐cell subset antibodies and a monoclonal anti‐HLA‐DR antibody. We found that the phenotype of Tar cells was OKT 3 + 4 + 8 + Dr − as determined by both indirect imnrunofluorescence microscopy and complement‐mediated killing of 51 Cr‐labelled Tar cells. In addition, we found that Tar lymphocytes were able to develop cytotoxicity against allogeneic and trinitrophenol (TNP)‐conjugated autologous target cells in the presence of interleukin‐2 (IL‐2) or serum thymic factor. However, these cells showed little or no cytotoxicity in the absence of interleukin‐2 or serum thymic factor. Tar lymphocytes generated helper function for B lymphocytes in the presence of interleukin‐2 in both pokeweed mitogen (PWM)‐ and purified protein derivative (PPD)‐stimulated cultures. Nevertheless, non‐IL‐2‐treated Tar cells did not exhibit any helper activity on B cells. Finally, pretreatment of Tar cells with 1000–1500 rad of X ray made these cells unable to develop helper function for B lymphocytes. It is concluded that: (1) OKT 3 + 4 + 8 + Dr − Tar cells are able to generate cytotoxicity against alloantigens and TNP‐labelled self structures provided they are stimulated by IL‐2 or serum thymic factor; (2) these cells need both to proliferate and to receive help from IL‐2 to develop helper cells capable of assisting B‐lymphocyte differentiation into plasma cells in both PWM‐ and PPD‐stimulated cultures.