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Detection of T‐Lymphocyte Subpopulations in the Peripheral Blood and the Synovium of Patients with Rheumatoid Arthritis and Juvenile Rheumatoid Arthritis Using Monoclonal Antibodies
Author(s) -
FØRRE Ø.,
THOEN J.,
DOBLOUG J. H.,
EGELAND T.,
KVIEN T. K.,
MELLBYE O. J.,
NATVIG J. B.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00642.x
Subject(s) - juvenile rheumatoid arthritis , synovial fluid , cytotoxic t cell , medicine , synovial membrane , immunology , rheumatoid arthritis , arthritis , monoclonal antibody , immunofluorescence , antigen , lymphocyte , t cell , pathology , antibody , immune system , biology , osteoarthritis , in vitro , biochemistry , alternative medicine
Monoclonal antibodies with specificities for various human T‐cell antigens were used in direct immunofluorescence to quantify the proportions of T lymphocytes with suppressor/ cytotoxic‐cell markers and with helper/inducer‐cell markers and of T lymphocytes with HLA‐DR antigens. Normal percentages of lymphocytes with suppressor/cytotoxic‐cell markers were detected in the peripheral blood synovial fluid and synovial tissue lymphocytes from patients with juvenile rheumatoid arthritis (JRA) and rheumatoid arthritis (RA), respectively. Normal percentages of T lymphocytes with helper/inducer‐cell markers were seen in the peripheral blood of RA and JRA patients and in the synovial tissues of RA patients. Slightly decreased percentages of cells with the helper/inducer‐cell marker were detected in the synovial fluids of JRA patients. The proportions of HLA‐DR‐positive T lymphocytes were highly increased in the synovial fluid and synovial tissue, whereas the numbers of these cells in the peripheral blood were normal. No significant differences in T γ cells were detected between peripheral blood, synovial fluid and synovial tissue of JRA patients or between peripheral blood and synovial tissue of RA patients.