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Enhancement of Human B Lymphocyte Differentiation in Vitro by Thyroid Hormone
Author(s) -
PAAVONEN T.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00640.x
Subject(s) - in vitro , thyroid , hormone , lymphocyte , endocrinology , biology , immunology , medicine , biochemistry
A study was made of the regulatory influence of a thyroid hormone, triiodothyronine (T 3 ), on human blood T‐ and B‐lymphocyte responses in vitro. Blood lymphocytes were stimulated with the T‐cell mitogens concanavalin A (Con A) and phytohaemagglutinin (PHA), with the T‐dependent B‐cell activator pokeweed mitogen (PWM), and with the T‐independ‐ent B‐cell activator Staphylococcus aureus Cowan I bacteria in the presence of various hormone concentrations. T 3 did not stimulate T‐cell proliferation. The number of immuno‐globulin‐containing and ‐secreting cells (plasmablasts) was increased in PWM and Staph. aureus cultures treated with T 3 . The maximal enhancement was reached at a concentration of 10 −9 ‐10 −7 M T 3 . Cell fractionation techniques revealed that T 3 apparently had a direct stimulatory effect on B‐cell differentiation.