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Endocytosis of Agarose in Mouse Peritoneal Macrophages in Vitro
Author(s) -
JOHNSON E.,
SELJELID R.,
BØGWALD J.,
LARM O.,
SCHOLANDER E.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00639.x
Subject(s) - endocytosis , agarose , in vitro , incubation , microbiology and biotechnology , antibody , chemistry , biology , immunology , biochemistry , receptor
We have studied the endocytosis of tritium‐labelled and of non‐radioactive agarose in mouse macrophages in vilro. The endocytosis was greatest and most rapid in syngeneic mouse serum and in human serum, reaching a plateau after 12 h of incubation. Ten per cent serum was the minimum concentration giving optimal ingestion. The endocytosis appeared to be regulated by mechanisms involving complement factors C3 and B. Different pretreatments of sera, inactivating or depleting C3 and B, resulted in 70–80% reduction of endocytosis, Preincubation of agarose in untreated serum increased the endocytosis of agarose in heat‐inactivated serum three‐fold, indicating that the essential factors were bound to agarose. Antibodies against C3 and B reduced endocytosis moderately hut significantly.