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The Lipid Nature of a Tumour‐Associated Autoantigen from a Chemically Induced Rat Hepatoma
Author(s) -
LANDO P.,
BERZINS K.,
PERLMANN P.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00637.x
Subject(s) - cardiolipin , phosphatidylethanolamine , antigen , phospholipid , cytotoxicity , thin layer chromatography , chemistry , biochemistry , antibody , microbiology and biotechnology , phospholipase a2 , epitope , cytotoxic t cell , chromatography , biology , membrane , in vitro , phosphatidylcholine , immunology , enzyme
The target antigens for the complement‐dependent cytotoxic antibodies in D23 tumour‐hearer serum from rats carrying the chemically induced D23 hepatoma were exlraciable from extranuclear membranes of tumours with chloroform methanol. The antigenic activity, measured by inhibition of D2J TBS cytotoxidly against D23 cells, was recovered in the phospholipid‐containing fraction after silica gel chromalography. Preparative thinlayer chromatography (in chloroform/methanol/0.25%., CaCl 2 ) revealed that the anligenie activity migrated similarly to phosphalidylethanolamine and cardiolipin, although these phospholipids did not cause the inhibition seen with the anligenic fractions, Phospholipase A 2 digestion of the phospholipid fraction did not affect the antigenic activity nor did it alter the mobility of the antigen when analysed with thin‐layer chromalography. From these results it was concluded that the antigen extracted from D23 hepatoma may he of phospholipid nature, although its molecular identity remains to be established.