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Concanavalin A Induces Suppressor Cell Activity in Both T gM and T non‐γ Cells: Most of the Suppressor Cells Do Not Carry HLA‐DR Antigens
Author(s) -
DOBLOUG J. H.,
FØRRE O.,
CHATTOPADHYAY C.,
NATVIG J. B.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00625.x
Subject(s) - concanavalin a , suppressor , stimulation , t cell , t lymphocyte , biology , antigen , cytotoxic t cell , interleukin 21 , microbiology and biotechnology , immunology , endocrinology , immune system , in vitro , biochemistry , gene
Concanavalin A (Con A) stimulation of T lymphocytes for 48 h induced a dose‐dependent increase in the percentage of T γ cells, and proliferation of these cells was dependent on pre‐existing T γ cells. Con A stimulation also induced a dose‐dependent increase in suppressor cell activity as measured in a Con A/mixed lymphocyte culture assay. Removal of T γ cells before stimulation with Con A did not influence suppressor cell activity, whereas depletion of T γ cells after Con A stimulation significantly reduced the suppression. Treatment of T lymphocytes with monclonal anti‐HLA‐DR antibodies and complement significantly increased the percentage of T γ cells and also increased the suppressor cell activity, both when the cells were treated before and when they were treated after Con A stimulation. Similarly, increased suppressor cell activity was also seen when Tγ cells were removed. The results indicate that Con‐A‐induced suppressor cells are recruited from both T γ and T non‐γ cells and also suggest that most of the Con‐A‐induced suppressor cell activity is exerted by HLA‐DR‐negative cells.