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V and C Genes in Creating Antibodies Directed to α(1→3)‐linked D‐Glucosyls in Dextran
Author(s) -
BELL C.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00623.x
Subject(s) - isotype , antibody , microbiology and biotechnology , antigenicity , kappa , beta (programming language) , chemistry , alpha (finance) , radioimmunoassay , immunoglobulin light chain , biology , immunology , biochemistry , medicine , monoclonal antibody , linguistics , philosophy , construct validity , nursing , patient satisfaction , computer science , programming language
An evaluation was made of the frequency of α(1→3)‐responsive, primary B precursors and their reactivity profiles in Igh‐l a1 (al) and Igh‐l b (b) allogroup mice, of dissimilar responsiveness as to quantity of the specific serum antibody produced, its light chain isotype, and its variable‐region, ligand‐modifiable antigenicity, to α(1→3) glucosyls in dextran B‐1355. al mice, exhibiting high λ 1 ‐specific serum antibody, with relatedness to the idiotypes (Id) shared by three BALB/c, α(1→3)‐specific, λ 1 myeloma proteins, possess specific primary splenic B precursors at a frequency of 1/5.5–7.3 × 104 cells; b mice, exhibiting low, K serum, Id − antibody, possess B splenic‐specific primary precursors at a frequency of 1/1.0–1.4 × 10 5 cells. A radioimmunoassay analysis showed correlations between the isotype profiles of the serum antibody response and of the primary precursors, namely μγ 1 in the al and μγ in the b allogroups, but also delineated minor primary μγλ 1 and μγκ precursors in al and μγκ and μλ 1 in b, at frequencies of 1/3 × 10 5 to 1/6.5 × 10 6 . The al, λ 1 precursors exhibited defined reactivity patterns for 3‐mono‐O‐3,6‐di‐O‐glucosyls and non‐reducing end groups in the B‐1355. The b, K precursors recognized the sequential residues.