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Studies on Human Epidermal Langerhans Cells
Author(s) -
BRAATHEN L. R.,
THORSBY E.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00621.x
Subject(s) - antigen , immunology , antigen presenting cell , human leukocyte antigen , in vitro , epidermis (zoology) , herpes simplex virus , biology , t cell , chemistry , microbiology and biotechnology , virus , immune system , biochemistry , anatomy
Epidermal cell suspensions were produced containing 3–6% HLA‐DR‐positive Langerhans cells. By using mixtures of epidermal cells and allogeneic T lymphocytes from donors sensitized to purified protein derivative of tuberculin (PPD) and herpes simplex virus (HSV), we could show that a proliferative in vitro response to these antigens, similar in strength to that seen in autologous combinations of macrophages or epidermal cells and T cells, could be obtained provided the epidermal cells shared both HLA‐D/DR determinants with the T‐cell donor. No antigen‐specific response was obtained in fully HLA‐D/DR‐disparate combinations. These results, together with previously reported data using other antigen‐presenting cells, further emphasize that one minimal requirement for antigen‐specific activation of T cells is co‐recognition of products of the self HLA‐D region.