Premium
Epstein‐Barr Virus Increases the Proliferative Response and the Generation of Suppressor and Cytotoxic T‐Cell Functions in Autologous Mixed Lymphocyte Reaction
Author(s) -
PALACIOS R.
Publication year - 1982
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1982.tb00617.x
Subject(s) - cytotoxic t cell , phytohaemagglutinin , biology , mixed lymphocyte reaction , pokeweed mitogen , antigen , interleukin 21 , immunology , epstein–barr virus , antigen presenting cell , monoclonal antibody , t cell , spleen , virology , peripheral blood mononuclear cell , antibody , virus , lymphocyte , immune system , in vitro , biochemistry
Epstein‐Barr virus (EBV) infection of stimulator cells significantly increased the proliferative response of T cells in the autologous mixed lymphocyte reaction (AMLR). The addition of a monoclonal anti‐HLA‐DR antibody toAMLR cultures in which either EBV‐infected or non‐infected non‐T cells wereused as stimulator cells strongly inhibited the proliferative response irrespective of the presence of EBV. It is concluded that EBV does not by itself activate the responding cells and that HLA‐DR antigens are necessary to trigger T cells. Increased generation of suppressor T cells, determined in both alloantigen‐induced DNA synthesis and pokeweed‐mitogen‐stimulated immunoglobulin production, was found after an EBV infection of stimulator cells. Similarly, EBV‐infected non‐T cells significantly increased the generation of killer T cells, determined in three different types of target cells: phytohaemagglutinin‐stimulated mononuclear cells, EBV‐transformed cells, and con‐canavalin‐A‐activated murine spleen cells. The increased T‐cell responses after an EBV infection may reflect the attempts in vivo to control andhold in check the viral infection.