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Age‐Dependent Allospecific Change in Susceptibility to Local Lymph Node GVHR in Rats
Author(s) -
HERON I.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00599.x
Subject(s) - lymph node , strain (injury) , in vivo , immunology , in vitro , lymph , inoculation , biology , physiology , andrology , pathology , medicine , genetics , anatomy
Inoculation of parental strain lymphocytes into the footpads of young F 1 hybrid rats induces GVHR, which can be quantitated by estimation of the weight of the popliteal lymph node 7 days later. In a number of strain combinations adult and old F 1 animals were also useful for the assay. In some, however, it was observed that aged F 1 rats did not support GVHR to the same extent as young did, when one of the parental strains served as donor for the GVHR, but reacted normally if the other parental strain was used. This phenomenon was analysed in serum and cell transfer experiments as well as in vitro in MLC. It was not possible to transfer suppression in vivo, whereas some degree of specific MLC inhibition was observed when serum from old F 1 animals undergoing GVHR was assessed. The ‘old’ pattern of response could be reverted partially by splenectomy and completely by low dose irradiation of the recipient before GVHR.