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Induction of Antigen‐Specific Immunological Unresponsiveness by Inhibitors of Human Lymphocyte‐Activating Factor
Author(s) -
BENDTZEN K.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00583.x
Subject(s) - antigen , lymphocyte , immunology , in vitro , peripheral blood mononuclear cell , chemistry , biology , biochemistry
Antigen‐stimulated blood mononuclear cells preincubated in vitro with Cd 2+ phenanthroline, a Zn 2+ ‐chelator, did not respond normally on restimulation with antigen, as judged by their ability to elaborate leucocyte migration inhibitory factor (LIF). Other divalent metal ions, including Zn 2+ , were ineffective. The effect was immunologically specific, since challenge of similarly pretreated cells with an unrelated antigen, to which the cells were also sensitized, resulted in normal LIF production. The lack of responsiveness could not be ascribed to cell death, carry‐over of the inhibitors, or exhaustive release of LIF during the inductive phase. Phenanthroline and Cd 2+ are known to inhibit the activity of the macrophage‐derived lymphocyte‐activating factor (LAF), whose effect is exerted non‐specifically on antigen‐stimulated lymphocytes in G 1 . Seen in the context of a two‐signal model of lymphocyte activation, it is hypothesized that immediate tolerance induction is triggered by delivery of the antigenic stimulus (signal 1) without addition of the nonspecific signal 2 (LAF).