Premium
ADCC of Cultured Human Melanoma Cells: Analysis with Monoclonal Antibodies to Human Melanoma‐associated Antigens
Author(s) -
IMAI K.,
NG A.K.,
GLASSY M. C.,
FERRONE S.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00577.x
Subject(s) - antibody dependent cell mediated cytotoxicity , monoclonal antibody , antigen , tunicamycin , microbiology and biotechnology , melanoma , cell culture , antibody , cycloheximide , lysis , biology , cell , chemistry , immunology , cancer research , apoptosis , biochemistry , protein biosynthesis , genetics , unfolded protein response
Four monoclonal antibodies to human melanoma‐associated antigens (MAA) were found to be able to mediate specific cell‐dependent lysis (ADCC) of cultured human melanoma cells. The extent of specific lysis of melanoma cells was influenced by the effector to target cell ratio, the amount of antibody added to the reaction mixture, and the incubation time. Cultured melanoma cells treated for 16 h with puromycin or cycloheximide (final concentration, 1.0 μg/ml) displayed increased Susceptibility to ADCC even though the cell surface expression of MAA was not changed. On the other hand, treatment of melanoma cells with tunicamycin (final concentration, 1.0 μg/ml) reduced the expression of MAA but did not affect susceptibility to ADCC. These findings suggest that other properties of the target cell membrane besides antigen density play a role in the outcome of ADCC reaction.