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Alpha‐Fetoprotein on Human Peripheral Blood Lymphocytes Does Not Block Complement‐dependent Lymphocytotoxicity
Author(s) -
REN E. C.,
CHAN S. H.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00569.x
Subject(s) - alpha fetoprotein , lymphocyte , sodium azide , cytotoxic t cell , immunofluorescence , peripheral blood , microbiology and biotechnology , chemistry , antigen , antibody , cytotoxicity , biology , immunology , hepatocellular carcinoma , biochemistry , cancer research , in vitro
Using a direct immunofluorescence assay, we showed that alpha‐fetoprotein (AFP) both in purified form and in hepatocellular carcinoma (HCC) sera was capable of binding onto 10–20% of T lymphocytes and 5–10% of B lymphocytes in human peripheral blood when these lymphocytes were preincubated in AFP‐positive fluids at 4°C in the presence of sodium azide. But when the preincubation temperature was raised to 37°C, most of the membrane‐bound AFP was internalized or shed, and, consequently, less than 3% of the cells showed positive membrane fluorescence. In addition, binding of AFP onto lymphocyte surface membrane and the continuous presence of large amounts of AFP in these lymphocyte cultures did not interfere with the action of cytotoxic antibodies directed against HLA determinants on the lymphocyte surface.