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Use of Plaque Assays to Study Thyroglobulin Autoantibody Synthesis by Human Peripheral Blood Lymphocytes
Author(s) -
McLACHLAN S. M.,
BIRD A. G.,
WEETMAN A. P.,
SMITH REES,
HALL R.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00560.x
Subject(s) - thyroglobulin , autoantibody , antibody , immunology , thyroiditis , thyroid , anti thyroid autoantibodies , medicine , peripheral blood , endocrinology
Plaque assays have been used to study thyroglobulin autoantibody synthesis and total immunoglobulin production by cultures of peripheral blood lymphocytes from patients with Hashimoto's thyroiditis. Freshly isolated Hashimoto or normal lymphocytes contained small numbers (7–1300) of total IgG plaque‐forming cells (PFC) and total IgM PFC, but specific thyroglobulin antibody PFC was undetectable. After 5–8 days' culture with pokeweed mitogen (PWM), total IgG and IgM PFC were markedly increased to a geometric mean of 10, 140 (8414–12,220) IgG PFC and 3450 (1663–7534) IgM PFC per 10 6 cultured lymphocytes (95% confidence limits in parentheses). Furthermore, a mean of 63 (22–287) specific IgG thyroglobulin antibody PFC per 10 6 lymphocytes were detectable in cultures of Hashimoto lymphocytes. The IgG thyroglobulin antibody PFC were stimulated by Epstein‐Barr virus (EBV) infection, suggesting that EBV infection may be useful in obtaining monoclonal thyroid autoantibodies.