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Clones of Human Cytotoxic T Lymphocytes Derived from an Allosensitized Individual: HLA Specificity and Cell Surface Markers
Author(s) -
MALISSEN B.,
KRISTENSEN T.,
GORIDIS C.,
MADSEN M.,
MAWAS C.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00558.x
Subject(s) - cytotoxic t cell , clone (java method) , human leukocyte antigen , serology , microbiology and biotechnology , cytolysis , biology , t cell , rosette (schizont appearance) , immunology , t lymphocyte , gel electrophoresis , polyacrylamide gel electrophoresis , cell , antigen , chemistry , dna , antibody , genetics , biochemistry , immune system , enzyme , in vitro
By planned immunization of a volunteer, two stable (≥6 months), specific, alloreactive cytolytic T‐cell clones have been established from his peripheral blood lymphocytes. One clone reacts with all serologically defined HLA‐Cw3 cells from our panel, whereas the other defines a split within the serological HLA‐B40 specificity. The two cytotoxic clones are SmIg‐negative, E‐rosette positive, EA and EAC rosette‐negative. HLA‐A, ‐B and ‐C‐positive, and also HLA‐DR‐ or ‘Ia like‐positive. In addition, they present very similar patterns of iodinated cell surface molecules as analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS‐PAGE), contrasting with that of an EBV cell line derived from the same donor.