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The Generation of Specific Cytotoxic Responses by Human Lymphocytes Requires Antigen and Activities Provided by a Lymphokine Supernatant‐
Author(s) -
WARREN H. S.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00185.x
Subject(s) - lymphokine , cytotoxic t cell , immunology , antigen , biology , genetics , in vitro
The requirements for the generation of specific cytotoxic responses by human lymphocytes have been investigated using as a model system the response to the ultraviolet light‐killed mouse tumour P815. These requirements are antigen (P815 uv ) and activities provided by a human lymphokine (LK) supernatant. Cells that give rise to non‐specific cytotoxicity can be separated on discontinuous Percoll density gradients after a 3‐day incubation of peripheral blood leucocytes with the lymphokine supernatant. Cells from which specific cytotoxic responses can subsequently be generated in the presence of P815 uv and LK sediment at a higher density.