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Colony Formation by Subpopulations of Human T Lymphocytes
Author(s) -
CLAËSSON M. H.,
SØNDERSTRUPHANSEN G.,
POULSEN P. BRIX
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00150.x
Subject(s) - biology , immunology
Phytohaemagglutinin‐induced human T‐lymphocyte colony formation in semisolid agar culture is the properly of erythrocyte rosette‐forming cells (E‐RFC) negative for the 7S IgG receptor (FcR − ). E‐RFC positive for the 7S IgG receptor (FcR + ), on the other hand, exhibit a limited capacity for colony formation and suppress colonies formed by FcR − E‐RFC. T colonies are composed of small lymphocytes and lymphoblasts, the vast majority being negative for the Fc receptor. Most colony cells (86%) carry the Leu 3a antigen, suggesting that they belong to the inducer/helper T‐cell subset, FcR + colony suppressor cells are small, slowly sedimenting cells (sedimentation velocity < 3.8 mm/h) and are strongly adherent to plastic, and their activity depends on the ability to synthesize DNA.