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Correlation between Suppression of B Cell Anti‐Hapten Response and Generation of Cytotoxic T Cells Induced by Hapten‐labelled Syngeneic Lymphocytes
Author(s) -
RAMOS T.,
MÖLLER E.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00118.x
Subject(s) - cytotoxic t cell , hapten , priming (agriculture) , cytotoxicity , spleen , microbiology and biotechnology , immune system , in vitro , in vivo , biology , immunology , chemistry , antibody , biochemistry , botany , germination
The correlation between suppression of B cell antibody response and generation of cytotoxic T cells induced by hapten (FITC) labelled syngeneic lymphocytes was studied. Primary in vitro sensitization with lymphocytes labelled with the hapten concentrations (FITC 0.5 and FITC 0.05) previously shown to induce specific B cell unresponsiveness generated cytotoxic cells that specifically lysed haptenated syngeneic blast cells. In vivo priming with either SC‐FITC 0.5 or SC‐FITC 0.05 could be demonstrated after in vitro restimulation with similarly haptenated cells. Thus, cells from primed mice gave a cytotoxic response already after 3 days in culture, whereas primary responses were only observed on day 5. Furthermore, immunization was evident as an increased specific lysis at low effector/target ratios. However, no direct cytotoxicity could be detected in spleen cells from immune animals. Comparison between kinetics of in vivo development of specific antibody suppression and induction of cytotoxic cells detectable after in vitro boosting revealed no correlation between the two phenomena.