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Indirect Haemagglutination for the Demonstration of Antibodies to Renal Antigens from Rats
Author(s) -
IVERSEN B. M.,
MATRE R.,
TØNDER O.
Publication year - 1981
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1981.tb00106.x
Subject(s) - hemagglutination , antibody , antigen , haemagglutination inhibition , hemagglutination tests , virology , immunology , medicine , biology , serology
An indirect haemagglutination (IHA) test using tanned sheep erythrocytes was developed to detect antibodies in sera from rabbits immunized with renal tubular epithelium (RTE) or with glomerular basement membrane (GBM) from rats. The RTK antigen preparation, called Fx1A, sensitized the erythrocytes directly, whereas the GBM preparation had to be treated with collagenase. The antisera to Fx1A gave titres up to 64,000, and the antisera to GBM up to several million. The sera were monospecific, since they gave no cross‐reaction in IHA, precipitation, or absorption tests using the IHA test, the quantitative aspects of experimental glomerulone‐phritis in rats can be studied. Preliminary results obtained in transfer experiments indicate a dose‐response relationship between the titra in IHA and the degree of proteinuria.