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Spontaneous Plaque‐forming Human Lymphocytes Detected with the Protein‐A Plaque Assay
Author(s) -
FREIJD A.,
KUNORI T.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00236.x
Subject(s) - virus quantification , antibody , immune system , adenoid , microbiology and biotechnology , immunology , biology , chemistry , virus
A sizable proportion of fresh human blood and adenoid lymphocytes were found to produce antibodies when tested in the protein‐A plaque assay of Gronowicz et al. Plaque formation was not caused by release of passively adsorbed immunoglobulin but depended on active‐ immunoglobulin secretion We have Investigated optimal conditions for plaque formation In this test system. Good reproducibility is obtained only if Cells to be tested are kept in the cold before assay, since the antibody‐forming capacity of these cells is rapidly exhausted at temperatures above 2°C. In blood lymphocytes the number of IgA‐producing cells exceeds those of IgG and IgM, whereat in the adenoid IgG plaque‐forming cell dominate. Studies on the on of this method for the monitoring of immune responses are in progress.