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T‐Lymphocyte Recognition of the I‐EC Gene Products (la) Requires Concomitant Recognition of I‐AB Gene Products
Author(s) -
PECK A. B.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00230.x
Subject(s) - major histocompatibility complex , antigen , biology , t lymphocyte , gene , microbiology and biotechnology , mixed lymphocyte reaction , lymphocyte , histocompatibility , gene product , immunology , genetics , t cell , human leukocyte antigen , immune system , gene expression
The murine primed lymphocyte typing (mPLT) assay, based on the sequential reactivation of specific immunocompetent, alloantigen‐reactive T blast cells in secondary mixed leucocyte culture (MLC), has been utilized to define the class II I‐EC‐subregion‐associated lymphocyte‐stimulating (LS) determinants of the major histocompatibility complex (MHC). The test panel of secondary stimulating cells has been expanded in this study to include the B10.W lines (mouse strains carrying MHC regions derived from wild mice). Data obtained using mPLT cells generated in primary MLC between I‐EC‐subregion‐disparate strain combinations reveal that(1) an absolute correlation between expression of the serologically defined la specificities and the capacity to induce subsequent secondary MLC activation does not exist, and (2) whereas the serologically defined la specificities appear to cluster on the α‐chain of the la molecule, T lymphocytes recognize either the β‐chain per se or an interaction product of the α‐chain plus β‐chains. Based on these observations, we conclude that T and B lymphocytes recognize different antigenic moieties expressed on the MHC class II antigens. How these data explain and/or affect several genetic concepts is discussed.