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A New Quantitative Fluorimetric Assay for Phagocytosis of Bacteria
Author(s) -
VRAY B.,
HOEBEKE J.,
SAINTGUILLAIN M.,
Leloup R.,
STROSBERG A. D.
Publication year - 1980
Publication title -
scandinavian journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.934
H-Index - 88
eISSN - 1365-3083
pISSN - 0300-9475
DOI - 10.1111/j.1365-3083.1980.tb00220.x
Subject(s) - bacteria , phagocytosis , lysis , lysozyme , fluorescein , microbiology and biotechnology , chemistry , fluorescence , chromatography , micrococcus , biology , paraformaldehyde , biochemistry , genetics , physics , quantum mechanics , organic chemistry
We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein‐labelled bacteria ( Micrococcus lysodeikticus ) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non‐phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme in phosphate‐buffered saline. After washing of the phagocytes, the fluorescence of those that have Ingested the labelled bacteria is. measured with a spectro fluorimeter. We report results obtained with different types of phagocytes which show that this method allows sensitivity, saturation and kinetic studies and the calculation of a phagocytic index.